Hotspot

psbE-petL

LSC · 68399-69842 · 1444 bp

Back to Hymenopus

Pi 0.014

MarkerSeek score 52.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1444
variable_sites: 20
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGCATTGCTGATATGGACCC	GGTTCAAGTCCTACAGAGCG	50.0	55.0	58.0	58.0	2371-2408 bp	1.0	81.2
psbE-petL_p2	TGCATTGCTGATATGGACCC	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.0	58.0	2425-2462 bp	1.0	81.2
psbE-petL_p3	TGGTCATTGGAGCCTCCTAA	GGTTCAAGTCCTACAGAGCG	50.0	55.0	58.0	58.0	2274-2311 bp	1.0	81.1
psbE-petL_p4	TGGTCATTGGAGCCTCCTAA	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.0	58.0	2328-2365 bp	1.0	81.1
psbE-petL_p5	TCGTTCGGGTTTGATTGTGT	GGTTCAAGTCCTACAGAGCG	45.0	55.0	58.0	58.0	2436-2473 bp	1.0	80.9