Hotspot

psbJ-psbL

LSC · 67695-67874 · 180 bp

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Pi 0.0222

MarkerSeek score 65.30

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 4
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	CCCAATCCGGAATATGACCC	ACACAATCAAACCCGAACGA	55.0	45.0	57.8	58.0	404-407 bp	1.0	78.8
psbJ-psbL_p2	TCTTCCAGTAGTATCGGCCA	ACACAATCAAACCCGAACGA	50.0	45.0	57.6	58.0	315-318 bp	1.0	77.1
psbJ-psbL_p3	GCCCAATCCGGAATATGACC	ACACAATCAAACCCGAACGA	55.0	45.0	58.5	58.0	405-408 bp	1.0	76.7
psbJ-psbL_p4	CCCAATCCGGAATATGACCC	GACACAATCAAACCCGAACG	55.0	50.0	57.8	57.7	405-408 bp	1.0	76.7
psbJ-psbL_p5	TGAGCCCAATCCGGAATATG	ACACAATCAAACCCGAACGA	50.0	45.0	57.4	58.0	408-411 bp	1.0	75.9