Hotspot

ndhC-trnV-UAC

LSC · 53200-54347 · 1148 bp

Back to Hymenopus

Pi 0.0135
MarkerSeek score 69.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1148
variable_sites
15
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATGGGGGATAAAACTCCGGA GCATGTTGGGTCTTTGAAGC 50.0 50.0 58.1 57.9 1346-1401 bp 1.0 81.4
ndhC-trnV-UAC_p2 CTGGACCTTTGGTAATGGGG GCATGTTGGGTCTTTGAAGC 55.0 50.0 57.9 57.9 1360-1415 bp 1.0 81.1
ndhC-trnV-UAC_p3 TCCGGAAATTCGAAATGCCA GCATGTTGGGTCTTTGAAGC 45.0 50.0 58.2 57.9 1332-1387 bp 1.0 80.9
ndhC-trnV-UAC_p4 ATGGGGGATAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1282-1337 bp 1.0 80.6
ndhC-trnV-UAC_p5 CTGGACCTTTGGTAATGGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.9 57.8 1296-1351 bp 1.0 80.2