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trnC-GCA-petN

LSC · 28728-29684 · 957 bp

Back to Hymenopus

Pi 0.00964

MarkerSeek score 64.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 957
variable_sites: 9
pi: 0.00964
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1005-1047 bp	1.0	79.8
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1041-1083 bp	1.0	78.4
trnC-GCA-petN_p3	CTGCAAATCCTTTTTCCCCG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.6	58.2	1035-1077 bp	1.0	78.3
trnC-GCA-petN_p4	GGGTGTCGCCTGATTAACAA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.2	57.6	1066-1108 bp	1.0	78.1
trnC-GCA-petN_p5	TCCTTTTTCCCCGGTTCAAA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.5	58.2	1028-1070 bp	1.0	77.6