Hotspot

trnH-GUG-psbA

LSC · 106-510 · 405 bp

Back to Hymenopus

Pi 0.0631

MarkerSeek score 87.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 25
pi: 0.0631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	531-540 bp	1.0	81.3
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	532-541 bp	1.0	81.1
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	532-541 bp	1.0	80.8
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.2	57.6	533-542 bp	1.0	80.6
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.7	58.3	524-533 bp	1.0	79.8