Hotspot

psbD-trnT-GGT

Genome · 41631-43153 · 1523 bp

Back to Hymenophyllum

Pi 0.0629
MarkerSeek score 86.60
Primer available yes
Label psbD-trnT-GGT

Feature Metadata

label_name
psbD-trnT-GGT
feature_type
igs
region
Genome
length_bp
1523
variable_sites
174
pi
0.0629
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.87
markerseek_score
86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGT_p1 CTCTTCTTAGCCAATCGTCCA GGCGTAAGTCATCGGTTCAA 47.6 50.0 57.5 58.3 1481-1634 bp 1.0 77.5
psbD-trnT-GGT_p2 TCTTCTTAGCCAATCGTCCA GGCGTAAGTCATCGGTTCAA 45.0 50.0 56.3 58.3 1480-1633 bp 1.0 75.9
psbD-trnT-GGT_p3 TCTCTTCTTAGCCAATCGTCC GGCGTAAGTCATCGGTTCAA 47.6 50.0 57.2 58.3 1482-1635 bp 1.0 75.3
psbD-trnT-GGT_p4 CTCTTCTTAGCCAATCGTCCA GCCATGGTAAGGCGTAAGTC 47.6 55.0 57.5 58.7 1491-1644 bp 1.0 74.2
psbD-trnT-GGT_p5 CGATCTCTTCTTAGCCAATCGT GGCGTAAGTCATCGGTTCAA 45.5 50.0 58.0 58.3 1485-1638 bp 1.0 73.5