Hotspot

psbM-trnD-GTC

Genome · 35834-36488 · 655 bp

Back to Hymenophyllum

Pi 0.0589

MarkerSeek score 86.00

Primer available yes

Label psbM-trnD-GTC

Feature Metadata

label_name: psbM-trnD-GTC
feature_type: igs
region: Genome
length_bp: 655
variable_sites: 73
pi: 0.0589
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GTC_p1	TGCAGTAGCTATGAATGCGA	GGTGCTCTAACCGATTGAACT	45.0	47.6	57.1	58.0	618-720 bp	1.0	76.7
psbM-trnD-GTC_p2	GTGCAGTAGCTATGAATGCG	GGTGCTCTAACCGATTGAACT	50.0	47.6	57.1	58.0	619-721 bp	1.0	76.3
psbM-trnD-GTC_p3	GCAGTAGCTATGAATGCGAGA	GGTGCTCTAACCGATTGAACT	47.6	47.6	58.0	58.0	617-719 bp	1.0	75.6
psbM-trnD-GTC_p4	TGCAGTAGCTATGAATGCGA	GGGATTGACGGGTTTCGAAC	45.0	55.0	57.1	59.2	662-764 bp	1.0	75.3
psbM-trnD-GTC_p5	TGCAGTAGCTATGAATGCGA	GGGATTGACGGGTTTCGAA	45.0	52.6	57.1	57.8	662-764 bp	1.0	75.0