Hotspot

atpH-atpI

Genome · 20608-21215 · 608 bp

Back to Hymenophyllum

Pi 0.0497

MarkerSeek score 86.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 608
variable_sites: 64
pi: 0.0497
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	GGAGAATCTATGGAAGGTCATCA	50.0	43.5	58.1	57.2	670-711 bp	1.0	58.9
atpH-atpI_p2	GCAGCAATAACAGAAGCAGC	AGGAGAATCTATGGAAGGTCA	50.0	42.9	58.1	55.2	671-712 bp	1.0	58.8
atpH-atpI_p3	GCAGCAATAACAGAAGCAGC	GCTGCAGCTTATATAGGAGA	50.0	45.0	58.1	54.1	685-726 bp	1.0	58.2
atpH-atpI_p4	CCGATAGAAGCAAGGCCTAC	GGAGAATCTATGGAAGGTCATCA	55.0	43.5	57.8	57.2	700-741 bp	1.0	58.0
atpH-atpI_p5	CCGATAGAAGCAAGGCCTAC	AGGAGAATCTATGGAAGGTCA	55.0	42.9	57.8	55.2	701-742 bp	1.0	57.9