Hotspot

atpF-atpH

Genome · 20015-20361 · 347 bp

Back to Hymenophyllum

Pi 0.0547

MarkerSeek score 83.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 347
variable_sites: 37
pi: 0.0547
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAAAACCCGCAGCAAGAATC	AGCATCATCATTTGCAAATCCC	50.0	40.9	58.3	57.9	428-439 bp	1.0	71.9
atpF-atpH_p2	CAAAACCCGCAGCAAGAATC	GCATCATCATTTGCAAATCCCT	50.0	40.9	58.3	57.9	427-438 bp	1.0	71.9
atpF-atpH_p3	CCCGCAGCAAGAATCCAATA	AGCATCATCATTTGCAAATCCC	50.0	40.9	58.3	57.9	423-434 bp	1.0	71.8
atpF-atpH_p4	CCCGCAGCAAGAATCCAATA	GCATCATCATTTGCAAATCCCT	50.0	40.9	58.3	57.9	422-433 bp	1.0	71.8
atpF-atpH_p5	CGCAGCAAGAATCCAATAGC	GCATCATCATTTGCAAATCCCT	50.0	40.9	57.6	57.9	420-431 bp	1.0	71.3