Hotspot

psbK-psbI

Genome · 13871-14675 · 805 bp

Back to Hymenophyllum

Pi 0.0655

MarkerSeek score 83.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 805
variable_sites: 100
pi: 0.0655
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCATCCAGAGCTGAAAAGGG	TTCGAATCCCTCTCTCTCCC	55.0	55.0	58.2	57.9	1582-1719 bp	0.8	80.3
psbK-psbI_p2	CCATCCAGAGCTGAAAAGGG	GGTTGGCCTTGAATTGAACG	55.0	50.0	58.2	57.9	2207-2210 bp	0.4	56.0
psbK-psbI_p3	TGGAAGATCCATCCAGAGCT	GGTTGGCCTTGAATTGAACG	50.0	50.0	57.8	57.9	2215 bp	0.2	46.0
psbK-psbI_p4	TGGAAGATCCATCCAGAGCT	TTCGAATCCCTCTCTCTCCC	50.0	55.0	57.8	57.9	1676 bp	0.2	45.9
psbK-psbI_p5	TGGAAGATCCATCCAGAGCT	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.8	58.2	1679 bp	0.2	44.8