Hotspot

ndhF

SSC · 129374-131617 · 2244 bp

Back to Hymenocallis

Pi 0.372

MarkerSeek score 77.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 834
pi: 0.372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCAATCAATCAGTTCCGCA	ACAGGAACAAGAGGGATCCA	45.0	50.0	57.5	58.0	2906 bp	0.5	55.7
ndhF_p2	TCCAATCAATCAGTTCCGCA	GGATCCACCGAACAAGACAA	45.0	50.0	57.5	57.8	2893 bp	0.5	54.7
ndhF_p3	TCCAATCAATCAGTTCCGCA	CAGGAACAAGAGGGATCCAC	45.0	55.0	57.5	57.6	2905 bp	0.5	52.8
ndhF_p4	ATCCAATCAATCAGTTCCGCA	ACAGGAACAAGAGGGATCCA	42.9	50.0	58.0	58.0	2907 bp	0.5	51.6
ndhF_p5	TCCAATCAATCAGTTCCGCA	CAGAAACGGAAGAAAGGGGA	45.0	50.0	57.5	57.4	2979 bp	0.5	51.6