Hotspot

rpl23-rpl2

IRa · 156331-156365 · 35 bp

Back to Hymenocallis

Pi 0.0286

MarkerSeek score 68.90

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 35
variable_sites: 1
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	CGCTTCAACCGGGTTATTCT	CACTTGACTGTCTACGGCTC	50.0	55.0	58.3	58.0	151 bp	1.0	78.3
rpl23-rpl2_p2	CGCTTCAACCGGGTTATTCT	CCACAACGATGCTGTCCATA	50.0	50.0	58.3	58.0	198 bp	1.0	78.1
rpl23-rpl2_p3	ATCATTACGCTTCAACCGGG	CACTTGACTGTCTACGGCTC	50.0	55.0	58.3	58.0	158 bp	1.0	77.7
rpl23-rpl2_p4	ATCATTACGCTTCAACCGGG	CCACAACGATGCTGTCCATA	50.0	50.0	58.3	58.0	205 bp	1.0	77.5
rpl23-rpl2_p5	CGCTTCAACCGGGTTATTCT	CTGTCTACGGCTCCATTACG	50.0	55.0	58.3	57.9	144 bp	1.0	77.5