Hotspot

rpl32-ndhF

SSC · 128433-129373 · 941 bp

Back to Hymenocallis

Pi 0.256

MarkerSeek score 79.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 941
variable_sites: 239
pi: 0.256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGCTTAGAACCAGCAGACAG	GGCCGAAAACACCATAGCTA	50.0	50.0	57.5	58.0	1427 bp	0.5	56.0
rpl32-ndhF_p2	TGCTTAGAACCAGCAGACAG	GAAGAGGGAATTGTGCGGAT	50.0	50.0	57.5	58.0	1998 bp	0.5	55.9
rpl32-ndhF_p3	TGCTTAGAACCAGCAGACAG	AGATTGGCCGAAAACACCAT	50.0	45.0	57.5	58.1	1432 bp	0.5	55.7
rpl32-ndhF_p4	TGCTTAGAACCAGCAGACAG	ACACTTGGAAAAGCCCACAT	50.0	45.0	57.5	57.9	1393 bp	0.5	55.6
rpl32-ndhF_p5	TGCTTAGAACCAGCAGACAG	AGCAACTGCACCGACAAATA	50.0	45.0	57.5	58.1	1976 bp	0.5	55.4