Hotspot

rbcL-accD

LSC · 58909-59719 · 811 bp

Back to Hymenocallis

Pi 0.0063

MarkerSeek score 64.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 5
pi: 0.0063
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	CCGGTATGTCCAAGAGCATT	50.0	50.0	57.9	57.7	1001-1013 bp	1.0	78.1
rbcL-accD_p2	CGCTGCTTGTGAAGTATGGA	CCGGTATGTCCAAGAGCATT	50.0	50.0	58.3	57.7	979-991 bp	1.0	76.9
rbcL-accD_p3	GCAAATGGAGCCCTGAACTA	CTCTTCACTTCCCCCGGTAT	50.0	55.0	57.9	58.5	1014-1026 bp	1.0	76.7
rbcL-accD_p4	AAATGGAGCCCTGAACTAGC	CCGGTATGTCCAAGAGCATT	50.0	50.0	57.6	57.7	999-1011 bp	1.0	75.7
rbcL-accD_p5	CGCTGCTTGTGAAGTATGGA	CTCTTCACTTCCCCCGGTAT	50.0	55.0	58.3	58.5	992-1004 bp	1.0	75.5