Hotspot

trnS-trnG

LSC · 8605-9173 · 569 bp

Back to Hymenocallis

Pi 0.143

MarkerSeek score 82.50

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 78
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	ATTAGCAATCCGCCGCTTTA	CCTTGGGATACAAATCGCGA	45.0	50.0	58.0	58.1	789-1442 bp	1.0	86.7
trnS-trnG_p2	ATTAGCAATCCGCCGCTTTA	CATCGTTAGCTTGGAAGGCT	45.0	50.0	58.0	58.0	1352-2005 bp	1.0	84.5
trnS-trnG_p3	ATTAGCAATCCGCCGCTTTA	CGTTAGCTTGGAAGGCTAGG	45.0	55.0	58.0	58.1	1349-2002 bp	1.0	84.2
trnS-trnG_p4	TTTGAATGCTCCGCTCATGA	CCTTGGGATACAAATCGCGA	45.0	50.0	57.9	58.1	1476-2126 bp	1.0	83.6
trnS-trnG_p5	TTTGAATGCTCCGCTCATGA	CATCGTTAGCTTGGAAGGCT	45.0	50.0	57.9	58.0	2039-2689 bp	1.0	82.6