Hotspot

ycf1

SSC · 125974-131412 · 5439 bp

Back to Hymenidium

Pi 0.0352

MarkerSeek score 71.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5439
variable_sites: 661
pi: 0.0352
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGTCATACCACCTTCAGT	GTTCTGTCCCCCAAAAACCT	50.0	50.0	58.0	57.9	1096-1126 bp	0.285714	57.1
ycf1_p2	TGGGTCATACCACCTTCAGT	CTTTGGTTCTGTCCCCCAAA	50.0	50.0	58.0	57.9	1101-1131 bp	0.285714	57.0
ycf1_p3	TGGGTCATACCACCTTCAGT	TCCGGCGAAACTAACCAAAA	50.0	45.0	58.0	58.0	842-872 bp	0.238095	54.5
ycf1_p4	TGGGTCATACCACCTTCAGT	TTTTAACGGCTTGGGGAACA	50.0	45.0	58.0	57.9	1135-1165 bp	0.238095	53.3
ycf1_p5	TGGGTCATACCACCTTCAGT	CTTCCCTTTGGTTCTGTCCC	50.0	55.0	58.0	58.1	1106-1136 bp	0.238095	53.2