Hotspot

rpl32-trnL-UAG

SSC · 115271-116232 · 962 bp

Back to Hymenidium

Pi 0.083

MarkerSeek score 81.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 962
variable_sites: 237
pi: 0.083
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	780-1110 bp	1.0	89.4
rpl32-trnL-UAG_p2	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	781-1111 bp	1.0	89.4
rpl32-trnL-UAG_p3	GCAGCCTTAAAAGCGTTGTC	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.3	58.1	1263-1600 bp	1.0	88.8
rpl32-trnL-UAG_p4	GAAAGGATATTGGGCAGCCT	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	793-1091 bp	0.47619	68.0
rpl32-trnL-UAG_p5	GAAAGGATATTGGGCAGCCT	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	794-1092 bp	0.47619	68.0