Hotspot

ndhF-rpl32

SSC · 114186-115105 · 920 bp

Back to Hymenidium

Pi 0.0735

MarkerSeek score 82.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 920
variable_sites: 216
pi: 0.0735
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCCGACGGAAAAAGAAGAA	CGGTTGGGTTGAAAGAGACA	45.0	50.0	58.0	58.0	1617-2005 bp	0.666667	78.0
ndhF-rpl32_p2	CCGTGTGAACCAAAACCCTA	CGGTTGGGTTGAAAGAGACA	50.0	50.0	58.0	58.0	2025-2413 bp	0.619048	76.2
ndhF-rpl32_p3	CCGTGTGAACCAAAACCCTA	TGTCGGTTGGGTTGAAAGAG	50.0	50.0	58.0	58.0	2028-2416 bp	0.619048	76.2
ndhF-rpl32_p4	GTTGCCGACGGAAAAAGAAG	CGGTTGGGTTGAAAGAGACA	50.0	50.0	58.0	58.0	1619-2007 bp	0.619048	76.2
ndhF-rpl32_p5	GTTGCCGACGGAAAAAGAAG	TGTCGGTTGGGTTGAAAGAG	50.0	50.0	58.0	58.0	1622-2010 bp	0.619048	76.2