Hotspot

petA-psbJ

LSC · 64420-65335 · 916 bp

Back to Hymenidium

Pi 0.0574
MarkerSeek score 83.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
916
variable_sites
175
pi
0.0574
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GTTCAATTGTCAGAAATGAATTTC CTGGATTGGGTTCATCTCTGT 29.2 47.6 53.8 57.4 936-1096 bp 1.0 52.0
petA-psbJ_p2 GTTCAATTGTCAGAAATGAATTTC TGGATTGGGTTCATCTCTGT 29.2 45.0 53.8 56.1 935-1095 bp 1.0 52.0
petA-psbJ_p3 GTTCAATTGTCAGAAATGAATTT CTGGATTGGGTTCATCTCTGT 26.1 47.6 52.5 57.4 936-1096 bp 1.0 52.0
petA-psbJ_p4 GTTCAATTGTCAGAAATGAATTTC TCTGGATTGGGTTCATCTCTG 29.2 47.6 53.8 57.1 937-1097 bp 1.0 52.0
petA-psbJ_p5 GTTCAATTGTCAGAAATGAATTT TGGATTGGGTTCATCTCTGT 26.1 45.0 52.5 56.1 935-1095 bp 1.0 52.0