Hotspot

ndhC-trnV-UAC

LSC · 51896-52627 · 732 bp

Back to Hymenidium

Pi 0.0818

MarkerSeek score 84.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 172
pi: 0.0818
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	728-1178 bp	0.952381	50.1
ndhC-trnV-UAC_p2	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	729-1179 bp	0.952381	50.1
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	728-1178 bp	0.952381	50.1
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	713-1163 bp	0.952381	50.1
ndhC-trnV-UAC_p5	CATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	33.3	55.0	52.3	58.1	727-1177 bp	0.952381	50.1