Hotspot

trnT-UGU-trnL-UAA

LSC · 48018-48823 · 806 bp

Back to Hymenidium

Pi 0.0595
MarkerSeek score 88.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
806
variable_sites
159
pi
0.0595
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.97
markerseek_score
88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 758-876 bp 0.952381 89.0
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 790-908 bp 0.952381 88.1
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG GCTCAATCCTTAAGTCCGCA 55.0 50.0 57.9 58.0 863-882 bp 0.142857 47.4
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG TCAATCCTTAAGTCCGCAGC 55.0 50.0 57.9 58.0 861-880 bp 0.142857 47.4
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT TCAATCCTTAAGTCCGCAGC 50.0 50.0 57.8 58.0 893-912 bp 0.142857 46.4