Hotspot

trnE-UUC-trnT-GGU

LSC · 31864-32756 · 893 bp

Back to Hymenidium

Pi 0.0831

MarkerSeek score 80.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 893
variable_sites: 210
pi: 0.0831
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTTAAAGGGC	50.0	50.0	57.8	57.9	682-983 bp	0.904762	86.0
trnE-UUC-trnT-GGU_p2	TTGTAGTACCCTACCCCCAG	TACCGCTGAGTTTAAAGGGC	55.0	50.0	57.4	57.9	717-1018 bp	0.857143	81.0
trnE-UUC-trnT-GGU_p3	CCGTTGCCTCCTTGAAAGAG	TACCGCTGAGTTTAAAGGGC	55.0	50.0	58.8	57.9	683-984 bp	0.904762	80.6
trnE-UUC-trnT-GGU_p4	AAACCACTAGACGATGGGGG	TACCGCTGAGTTTAAAGGGC	55.0	50.0	59.1	57.9	654-955 bp	0.904762	78.6
trnE-UUC-trnT-GGU_p5	TTTGTAGTACCCTACCCCCA	TACCGCTGAGTTTAAAGGGC	50.0	50.0	57.0	57.9	718-1019 bp	0.714286	71.4