Hotspot

petN-psbM

LSC · 29884-30629 · 746 bp

Back to Hymenidium

Pi 0.0534

MarkerSeek score 81.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 118
pi: 0.0534
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TGGCTGAGTCTTGAATTCCG	CTTTCCCTTTTCGTTTCGCC	50.0	50.0	57.9	58.0	1840-2410 bp	0.666667	77.5
petN-psbM_p2	GGCTGAGTCTTGAATTCCGT	CTTTCCCTTTTCGTTTCGCC	50.0	50.0	57.9	58.0	1839-2409 bp	0.666667	77.5
petN-psbM_p3	TGGCTGAGTCTTGAATTCCG	CCCTTTTCGTTTCGCCTTTC	50.0	50.0	57.9	58.0	1836-2286 bp	0.47619	67.9
petN-psbM_p4	GGCTGAGTCTTGAATTCCGT	CCCTTTTCGTTTCGCCTTTC	50.0	50.0	57.9	58.0	1835-2285 bp	0.47619	67.8
petN-psbM_p5	AGTCCCTTTTTGACTCTGCG	CCCTTTTCGTTTCGCCTTTC	50.0	50.0	58.1	58.0	1456-1813 bp	0.238095	55.2