Hotspot

trnC-GCA-petN

LSC · 29087-29793 · 707 bp

Back to Hymenidium

Pi 0.0409

MarkerSeek score 83.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 97
pi: 0.0409
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	636-773 bp	1.0	90.1
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	654-791 bp	0.952381	86.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	635-772 bp	1.0	84.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	644-781 bp	1.0	84.1
trnC-GCA-petN_p5	CCAGTTCAAATCCGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.8	58.2	631-768 bp	1.0	84.1