Hotspot

trnS-GCU-trnG-UCC

LSC · 9031-9567 · 537 bp

Back to Hymenidium

Pi 0.0627

MarkerSeek score 84.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 112
pi: 0.0627
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	585-818 bp	1.0	60.4
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	580-813 bp	1.0	59.6
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	AACGAATCACACTTTTACCACT	50.0	36.4	57.6	56.2	586-819 bp	1.0	58.5
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	AACGAATCACACTTTTACCACT	55.0	36.4	58.5	56.2	581-814 bp	1.0	57.7
trnS-GCU-trnG-UCC_p5	TAGTCCACTCAGCCATCTCT	ACGAATCACACTTTTACCACT	50.0	38.1	57.2	55.5	581-814 bp	1.0	57.0