Hotspot

ycf1

SSC · 120592-124530 · 3939 bp

Back to Hymenasplenium

Pi 0.0448

MarkerSeek score 72.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3939
variable_sites: 526
pi: 0.0448
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAACTGGTTAACTCGCCGT	GCACATCTGGCTCGAAGTAA	45.0	50.0	58.0	58.0	1446-1464 bp	0.916667	84.2
ycf1_p2	AAAACTGGTTAACTCGCCGT	TTATTGAAGGGGTCCATGCG	45.0	50.0	58.0	57.9	1269-1287 bp	0.916667	83.9
ycf1_p3	AAAACTGGTTAACTCGCCGT	AGTCAAAGCTTGCTCGTTCA	45.0	45.0	58.0	58.0	1028-1043 bp	0.833333	80.6
ycf1_p4	AAAACTGGTTAACTCGCCGT	TCAGTCAAAGCTTGCTCGTT	45.0	45.0	58.0	58.0	1030-1045 bp	0.833333	80.6
ycf1_p5	AAAACTGGTTAACTCGCCGT	TTGTTTGCCGACTCGAAAGA	45.0	45.0	58.0	58.1	1113 bp	0.25	51.0