Hotspot

ycf1-chlN

SSC · 124531-126071 · 1541 bp

Back to Hymenasplenium

Pi 0.0598

MarkerSeek score 78.90

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: SSC
length_bp: 1541
variable_sites: 259
pi: 0.0598
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	GAGCTTTCGCCGGTAAAGTA	TGCGAGAAGATGAAGAAGCC	50.0	50.0	58.0	58.0	2073-2092 bp	1.0	88.1
ycf1-chlN_p2	ATTTTGTGCTGCAGTACCCA	ATGCGGAGTGAACCCTTTTT	45.0	45.0	58.0	58.0	2714-2727 bp	0.833333	81.7
ycf1-chlN_p3	TTACTTCGAGCCAGATGTGC	ATGCGGAGTGAACCCTTTTT	50.0	45.0	58.0	58.0	2747-2760 bp	0.833333	81.7
ycf1-chlN_p4	GAGCTTTCGCCGGTAAAGTA	ATGCGGAGTGAACCCTTTTT	50.0	45.0	58.0	58.0	2437-2456 bp	0.833333	81.5
ycf1-chlN_p5	GGTTCTTCGTCGCAATCTCT	ATGCGGAGTGAACCCTTTTT	50.0	45.0	58.0	58.0	2397-2410 bp	0.583333	71.3