Hotspot

ccsA-ndhD

SSC · 112205-112608 · 404 bp

Back to Hymenasplenium

Pi 0.0863

MarkerSeek score 79.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 404
variable_sites: 99
pi: 0.0863
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCTCCGCAACTTTAGGTCTC	AGGATATGGGCTGATTCGGA	55.0	50.0	58.0	58.0	1745-1760 bp	0.583333	72.1
ccsA-ndhD_p2	CTCCGCAACTTTAGGTCTCC	AGGATATGGGCTGATTCGGA	55.0	50.0	58.0	58.0	1744-1759 bp	0.583333	72.1
ccsA-ndhD_p3	CTCCGCAACTTTAGGTCTCC	ACGTTTAGTGCATTCTCGCT	55.0	45.0	58.0	57.9	1386-1401 bp	0.583333	72.0
ccsA-ndhD_p4	TTGATGCGCGGAATTTTTCC	ACGTTTAGTGCATTCTCGCT	45.0	45.0	58.0	57.9	1152-1156 bp	0.333333	57.0
ccsA-ndhD_p5	TTGATGCGCGGAATTTTTCC	AGGATATGGGCTGATTCGGA	45.0	50.0	58.0	58.0	1510-1514 bp	0.333333	56.9