Hotspot

rpl32-trnP-GGG

SSC · 110213-110640 · 428 bp

Back to Hymenasplenium

Pi 0.111

MarkerSeek score 78.20

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: SSC
length_bp: 428
variable_sites: 122
pi: 0.111
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	GGAAGTATGGGCCGATTTGT	GGATTCTTCAAAACTGCCGC	50.0	50.0	57.9	58.0	1201-1225 bp	0.666667	78.1
rpl32-trnP-GGG_p2	AGGGAAGATTTCACGATGCC	GGATTCTTCAAAACTGCCGC	50.0	50.0	58.0	58.0	1440-1464 bp	0.5	71.2
rpl32-trnP-GGG_p3	GGAAGTATGGGCCGATTTGT	GTGCTAGTTGTATGACGCCA	50.0	50.0	57.9	58.0	908-916 bp	0.25	53.8
rpl32-trnP-GGG_p4	GAGACTTTGCCCCAATCCAA	GTGCTAGTTGTATGACGCCA	50.0	50.0	58.1	58.0	1126-1134 bp	0.25	52.9
rpl32-trnP-GGG_p5	AGGGAAGATTTCACGATGCC	GTGCTAGTTGTATGACGCCA	50.0	50.0	58.0	58.0	1155 bp	0.083333	42.9