Hotspot

trnI-GAU-rrn16S

IRb · 91586-91893 · 308 bp

Back to Hymenasplenium

Pi 0.068
MarkerSeek score 80.30
Primer available yes
Label trnI-GAU-rrn16S

Feature Metadata

label_name
trnI-GAU-rrn16S
feature_type
igs
region
IRb
length_bp
308
variable_sites
48
pi
0.068
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16S_p1 CTACCGCTGAGCTAATAGCC AGTGACTGGAGTGAAGTCGT 55.0 50.0 57.6 58.3 380-394 bp 1.0 85.8
trnI-GAU-rrn16S_p2 CTACCGCTGAGCTAATAGCC AAGTCGTAACAAGGTAGCCG 55.0 50.0 57.6 57.6 367-381 bp 1.0 85.5
trnI-GAU-rrn16S_p3 CTACCGCTGAGCTAATAGCC GGCTAGTGACTGGAGTGAAG 55.0 55.0 57.6 57.3 384-398 bp 1.0 82.9
trnI-GAU-rrn16S_p4 CTACCGCTGAGCTAATAGCC GGGCTAGTGACTGGAGTGAA 55.0 55.0 57.6 58.7 385-399 bp 1.0 82.3
trnI-GAU-rrn16S_p5 GAAGTTTCCACAGTCCAGGC AGTGACTGGAGTGAAGTCGT 55.0 50.0 58.8 58.3 428-442 bp 1.0 82.2