Hotspot

ndhC-trnM-CAU

LSC · 49284-50486 · 1203 bp

Back to Hymenasplenium

Pi 0.0561

MarkerSeek score 84.90

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 190
pi: 0.0561
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	TGCGTGAACCAAACCAACTA	TATTGCGCCATCCGAAGAAA	45.0	45.0	58.0	58.0	1728-1751 bp	0.916667	87.1
ndhC-trnM-CAU_p2	ACTTCTCTGGTCCCTTTCGA	TATTGCGCCATCCGAAGAAA	50.0	45.0	58.0	58.0	1516-1539 bp	0.833333	83.9
ndhC-trnM-CAU_p3	TTCATAGTTACGGTGCCAGC	TATTGCGCCATCCGAAGAAA	50.0	45.0	58.0	58.0	2065-2088 bp	0.833333	83.2
ndhC-trnM-CAU_p4	CAGAAGCCGAAGCAAACATG	TATTGCGCCATCCGAAGAAA	50.0	45.0	58.0	58.0	1620-1626 bp	0.416667	61.7
ndhC-trnM-CAU_p5	GACGTCAAAAACCGCAGAAG	TATTGCGCCATCCGAAGAAA	50.0	45.0	58.0	58.0	1634-1636 bp	0.25	51.7