Hotspot

petN-trnC-GCA

LSC · 29356-29845 · 490 bp

Back to Hymenasplenium

Pi 0.0544

MarkerSeek score 84.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 78
pi: 0.0544
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	GCCCAAGCTATAGTAACTAAGTCC	CAGTTCGAGTCTGGGTGTC	45.8	57.9	58.5	57.5	534-575 bp	1.0	51.4
petN-trnC-GCA_p2	CCCAAGCTATAGTAACTAAGTCCA	CAGTTCGAGTCTGGGTGTC	41.7	57.9	57.3	57.5	533-574 bp	1.0	51.4
petN-trnC-GCA_p3	GCCCAAGCTATAGTAACTAAGTC	CAGTTCGAGTCTGGGTGTC	43.5	57.9	56.3	57.5	534-575 bp	1.0	51.4
petN-trnC-GCA_p4	GCCCAAGCTATAGTAACTAAGTCC	CCAGTTCGAGTCTGGGTGT	45.8	57.9	58.5	58.9	535-576 bp	1.0	51.4
petN-trnC-GCA_p5	GCCCAAGCTATAGTAACTAAGT	CAGTTCGAGTCTGGGTGTC	40.9	57.9	55.1	57.5	534-575 bp	1.0	51.4