Hotspot

trnS-GCU-ycf12

LSC · 7931-8441 · 511 bp

Back to Hymenasplenium

Pi 0.065

MarkerSeek score 82.20

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 83
pi: 0.065
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	AGACCTCTCAGCCATCTCTC	AGTTGCACAATTATTTCTAGATTCA	55.0	28.0	57.9	55.3	546-565 bp	1.0	50.2
trnS-GCU-ycf12_p2	AGACCTCTCAGCCATCTCTC	AAGTTGCACAATTATTTCTAGATTCA	55.0	26.9	57.9	56.0	547-566 bp	1.0	50.2
trnS-GCU-ycf12_p3	AGACCTCTCAGCCATCTCTC	GTTGCACAATTATTTCTAGATTCA	55.0	29.2	57.9	54.0	545-564 bp	1.0	50.2
trnS-GCU-ycf12_p4	AGACCTCTCAGCCATCTCTC	AGAAAGTTGCACAATTATTTCT	55.0	27.3	57.9	52.6	550-569 bp	1.0	50.1
trnS-GCU-ycf12_p5	AGACCTCTCAGCCATCTCTC	AGAAAGTTGCACAATTATTTCTAGA	55.0	28.0	57.9	55.3	550-569 bp	1.0	50.1