Hotspot

trnQ-UUG-psbK

LSC · 6534-6871 · 338 bp

Back to Hymenasplenium

Pi 0.0491

MarkerSeek score 79.10

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 338
variable_sites: 47
pi: 0.0491
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CGAAGAAGCTCGATCCACAA	CTAACTGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1143-1151 bp	0.5	64.1
trnQ-UUG-psbK_p2	CGAAGAAGCTCGATCCACAA	CGTAAGGGAAGGGAATGAGC	50.0	55.0	58.0	58.0	1219-1227 bp	0.25	52.6
trnQ-UUG-psbK_p3	CGAAGAAGCTCGATCCACAA	GTAAGGGAAGGGAATGAGCG	50.0	55.0	58.0	58.0	1218-1226 bp	0.25	52.6
trnQ-UUG-psbK_p4	TTCCAAAGTTCTATCCGCCG	CGTAAGGGAAGGGAATGAGC	50.0	55.0	58.0	58.0	1300-1308 bp	0.25	52.4
trnQ-UUG-psbK_p5	TTCCAAAGTTCTATCCGCCG	GTAAGGGAAGGGAATGAGCG	50.0	55.0	58.0	58.0	1299-1307 bp	0.25	52.4