Pi
0.03
MarkerSeek score
73.30
Primer available
yes
Label
rrn4.5
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rrn4.5_p1 | GGATGGAGCGACAGAAGTTT | CGAGTGCCTGATCGAATTGA | 50.0 | 50.0 | 57.9 | 58.1 | 173-177 bp | 1.0 | 81.8 |
| rrn4.5_p2 | TCTCCTATTCCGACTTCCCC | CGAGTGCCTGATCGAATTGA | 55.0 | 50.0 | 57.9 | 58.1 | 244-248 bp | 1.0 | 81.0 |
| rrn4.5_p3 | GGATGGAGCGACAGAAGTTT | GATAGAGAGGGAGGGCAGAG | 50.0 | 60.0 | 57.9 | 58.1 | 241-245 bp | 1.0 | 80.9 |
| rrn4.5_p4 | TCTCCTATTCCGACTTCCCC | GATAGAGAGGGAGGGCAGAG | 55.0 | 60.0 | 57.9 | 58.1 | 312-316 bp | 1.0 | 80.5 |
| rrn4.5_p5 | CCTATTCCGACTTCCCCAGA | CGAGTGCCTGATCGAATTGA | 55.0 | 50.0 | 58.2 | 58.1 | 241-245 bp | 1.0 | 80.0 |
| rrn4.5_p1 | CGAGTGCCTGATCGAATTGA | GGATGGAGCGACAGAAGTTT | 50.0 | 50.0 | 58.1 | 57.9 | 173-177 bp | 1.0 | 81.8 |
| rrn4.5_p2 | CGAGTGCCTGATCGAATTGA | TCTCCTATTCCGACTTCCCC | 50.0 | 55.0 | 58.1 | 57.9 | 244-248 bp | 1.0 | 81.0 |
| rrn4.5_p3 | GATAGAGAGGGAGGGCAGAG | GGATGGAGCGACAGAAGTTT | 60.0 | 50.0 | 58.1 | 57.9 | 241-245 bp | 1.0 | 80.9 |
| rrn4.5_p4 | GATAGAGAGGGAGGGCAGAG | TCTCCTATTCCGACTTCCCC | 60.0 | 55.0 | 58.1 | 57.9 | 312-316 bp | 1.0 | 80.5 |
| rrn4.5_p5 | CGAGTGCCTGATCGAATTGA | CCTATTCCGACTTCCCCAGA | 50.0 | 55.0 | 58.1 | 58.2 | 241-245 bp | 1.0 | 80.0 |