Hotspot

trnN-GUU-trnR-ACG

IRa · 135284-136101 · 818 bp

Back to Hymenaea

Pi 0.0195

MarkerSeek score 74.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 818
variable_sites: 15
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	1000-1003 bp	1.0	82.5
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	923-926 bp	1.0	82.4
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	910-913 bp	1.0	82.3
trnN-GUU-trnR-ACG_p4	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	1037-1040 bp	1.0	81.8
trnN-GUU-trnR-ACG_p5	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	999-1002 bp	1.0	81.8