Hotspot

ndhG-ndhI

SSC · 124156-124642 · 487 bp

Back to Hymenaea

Pi 0.0234

MarkerSeek score 73.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 487
variable_sites: 11
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	689-694 bp	1.0	82.0
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAA	TTTGGGCCGTTTACCAATGT	45.0	45.0	57.9	58.3	678-683 bp	1.0	80.5
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAA	CTTTGGGCCGTTTACCAATG	45.0	50.0	57.9	57.6	679-684 bp	1.0	79.9
ndhG-ndhI_p4	TCCCAACGAAAAGGCAGAAA	CAAATTGCTTTGGGCCGTTT	45.0	45.0	57.9	58.4	686-691 bp	1.0	79.5
ndhG-ndhI_p5	TCCCAACGAAAAGGCAGAAA	AATTGCTTTGGGCCGTTTAC	45.0	45.0	57.9	57.6	684-689 bp	1.0	79.2