Hotspot

ndhF-rpl32

SSC · 117024-118161 · 1138 bp

Back to Hymenaea

Pi 0.0172

MarkerSeek score 72.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1138
variable_sites: 18
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCATCGGAAAAAGGA	AGAAAGAGATGTGGCTAACGA	45.0	42.9	58.0	56.8	1309-1354 bp	1.0	67.1
ndhF-rpl32_p2	TGTTGCCATCGGAAAAAGGA	AGAAAGAGATGTGGCTAACGAA	45.0	40.9	58.0	57.5	1309-1354 bp	1.0	64.7
ndhF-rpl32_p3	CCAAACACGGCGAAGATTTT	AGAAAGAGATGTGGCTAACGA	45.0	42.9	57.6	56.8	1330-1375 bp	1.0	63.8
ndhF-rpl32_p4	CGGAAAAAGGAGAAGTCCCA	AGAAAGAGATGTGGCTAACGA	50.0	42.9	57.4	56.8	1300-1345 bp	1.0	62.7
ndhF-rpl32_p5	TGTTGCCATCGGAAAAAGGA	GAAAGAGATGTGGCTAACGA	45.0	45.0	58.0	55.2	1308-1353 bp	1.0	62.6