Hotspot

rpl14-rpl16

LSC · 85444-85591 · 148 bp

Back to Hymenaea

Pi 0.028

MarkerSeek score 71.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 4
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCGGCTACATTCAAATGGGT	AGCAGCATCCAAAATGCCTA	45.0	45.0	57.2	57.9	227-232 bp	1.0	75.6
rpl14-rpl16_p2	TCGGCTACATTCAAATGGGT	TAGCAGCATCCAAAATGCCT	45.0	45.0	57.2	57.9	228-233 bp	1.0	75.5
rpl14-rpl16_p3	CCCGCTATTATCGGCTACAT	AGCAGCATCCAAAATGCCTA	50.0	45.0	57.0	57.9	237-242 bp	1.0	74.1
rpl14-rpl16_p4	CCCGCTATTATCGGCTACAT	TAGCAGCATCCAAAATGCCT	50.0	45.0	57.0	57.9	238-243 bp	1.0	74.1
rpl14-rpl16_p5	ATACACATCAATTCGCGGGC	AGCAGCATCCAAAATGCCTA	50.0	45.0	59.1	57.9	257-262 bp	1.0	73.1