Hotspot

rps11-rpl36

LSC · 83271-83814 · 544 bp

Back to Hymenaea

Pi 0.0495

MarkerSeek score 89.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 26
pi: 0.0495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 89.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTCTACGTGAACG	CGTAGGCGAGGACGAATTAT	50.0	50.0	57.0	57.3	651-660 bp	1.0	71.6
rps11-rpl36_p2	CCCTTTGGTATTCTACGCACA	CGTAGGCGAGGACGAATTAT	47.6	50.0	58.0	57.3	690-699 bp	1.0	71.4
rps11-rpl36_p3	TCCCTTTGGTATTCTACGCAC	CGTAGGCGAGGACGAATTAT	47.6	50.0	57.7	57.3	691-700 bp	1.0	69.5
rps11-rpl36_p4	CGTCCATTTCTACGTGAACG	AAGGGCCTCTGTTCGTAAAA	50.0	45.0	57.0	57.1	694-703 bp	1.0	69.3
rps11-rpl36_p5	CCCTTTGGTATTCTACGCACA	AAGGGCCTCTGTTCGTAAAA	47.6	45.0	58.0	57.1	733-742 bp	1.0	69.2