Hotspot

rbcL-accD

LSC · 59989-61236 · 1248 bp

Back to Hymenaea

Pi 0.0242
MarkerSeek score 68.10
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
1248
variable_sites
25
pi
0.0242
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGTCCTGAATTAGCTGCTGC TTAGTTAGCCCACACCCGTA 50.0 50.0 58.0 58.1 1182-1391 bp 1.0 84.8
rbcL-accD_p2 ATCCGTCAGGCTAGCAAATG TTAGTTAGCCCACACCCGTA 50.0 50.0 58.0 58.1 1203-1412 bp 1.0 84.7
rbcL-accD_p3 AGTCCTGAATTAGCTGCTGC TAGTTAGCCCACACCCGTAT 50.0 50.0 58.0 57.8 1181-1390 bp 1.0 84.0
rbcL-accD_p4 ATCCGTCAGGCTAGCAAATG TAGTTAGCCCACACCCGTAT 50.0 50.0 58.0 57.8 1202-1411 bp 1.0 83.9
rbcL-accD_p5 AGTCCTGAATTAGCTGCTGC GCCCACACCCGTATTCTAAC 50.0 55.0 58.0 58.3 1175-1384 bp 1.0 82.6