Hotspot

psaA-ycf3

LSC · 45307-46132 · 826 bp

Back to Hymenaea

Pi 0.01

MarkerSeek score 67.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 8
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	957-973 bp	1.0	81.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	963-979 bp	1.0	81.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACAGGGC	50.0	50.0	57.9	58.1	921-937 bp	1.0	81.3
psaA-ycf3_p4	GAAAAATGACCGGGTTTGGC	AGCCCTTACCCCTGGTAATT	50.0	50.0	58.2	58.0	975-991 bp	1.0	81.1
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	930-946 bp	1.0	81.1