Hotspot

psbZ-trnG-GCC

LSC · 39057-39786 · 730 bp

Back to Hymenaea

Pi 0.0149

MarkerSeek score 68.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 10
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	883-884 bp	1.0	83.4
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	886-887 bp	1.0	81.9
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	879-880 bp	1.0	81.7
psbZ-trnG-GCC_p4	CTTCTCCTGATGGTTGGTCG	GGATAGCGGGAATTGAACCC	55.0	55.0	58.0	58.4	904-905 bp	1.0	81.6
psbZ-trnG-GCC_p5	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	884-885 bp	1.0	81.5