Hotspot

atpH-atpI

LSC · 14463-15955 · 1493 bp

Back to Hymenaea

Pi 0.027

MarkerSeek score 75.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1493
variable_sites: 40
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1614-1799 bp	1.0	81.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1580-1765 bp	1.0	81.2
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.0	58.3	1663-1848 bp	1.0	80.1
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1629-1814 bp	1.0	80.1
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1581-1766 bp	1.0	80.0