Hotspot

atpF-atpH

LSC · 13401-14216 · 816 bp

Back to Hymenaea

Pi 0.0205

MarkerSeek score 69.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 13
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCGAAACTCCCAGC	AGGCAGAGGGAAAAATACGG	50.0	50.0	58.1	57.6	893-1000 bp	1.0	80.9
atpF-atpH_p2	CGTATGGCCAGTAACCCAAA	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	874-981 bp	1.0	80.8
atpF-atpH_p3	TTAAACCCGAAACTCCCAGC	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	58.1	57.5	897-1004 bp	1.0	80.1
atpF-atpH_p4	CGTATGGCCAGTAACCCAAA	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	878-985 bp	1.0	80.0
atpF-atpH_p5	TTAAACCCGAAACTCCCAGC	GCAGAGGGAAAAATACGGGG	50.0	55.0	58.1	58.6	891-998 bp	1.0	79.4