Hotspot

trnS-GCU-trnG-UCC

LSC · 8433-9089 · 657 bp

Back to Hymenaea

Pi 0.031

MarkerSeek score 78.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 20
pi: 0.031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACGAGGGACCC	50.0	50.0	58.0	57.6	770-973 bp	1.0	79.5
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACGAGGGACCC	50.0	50.0	58.0	57.6	769-972 bp	1.0	79.5
trnS-GCU-trnG-UCC_p3	CAGATTAGCAATCCGACGCT	ATGAATCAAACGAGGGACCC	50.0	50.0	58.1	57.6	778-981 bp	1.0	78.4
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACGAGGGACCC	50.0	50.0	58.2	57.6	824-1027 bp	1.0	78.2
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	ATGAATCAAACGAGGGACCC	50.0	50.0	58.4	57.6	772-975 bp	1.0	76.7