Hotspot

rps15-ycf1

SSC · 121398-121800 · 403 bp

Back to Hylotelephium

Pi 0.0107
MarkerSeek score 52.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
403
variable_sites
11
pi
0.0107
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACGGATCCCTTGGTTTC TTCTTTGGCCGACTTGTCAA 50.0 45.0 57.8 58.0 540-599 bp 1.0 83.6
rps15-ycf1_p2 TCAACGGATCCCTTGGTTTC TTTCTTTGGCCGACTTGTCA 50.0 45.0 57.8 58.0 541-600 bp 1.0 83.6
rps15-ycf1_p3 CAACGGATCCCTTGGTTTCT TTCTTTGGCCGACTTGTCAA 50.0 45.0 57.8 58.0 539-598 bp 1.0 83.5
rps15-ycf1_p4 CAACGGATCCCTTGGTTTCT TTTCTTTGGCCGACTTGTCA 50.0 45.0 57.8 58.0 540-599 bp 1.0 83.5
rps15-ycf1_p5 TCAACGGATCCCTTGGTTTC TCTTTGGCCGACTTGTCAAT 50.0 45.0 57.8 57.7 539-598 bp 1.0 81.8