Hotspot

trnL-UAG-ccsA

SSC · 112440-112510 · 71 bp

Back to Hylotelephium

Pi 0.0302

MarkerSeek score 56.60

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 71
variable_sites: 5
pi: 0.0302
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CTTGAGCATCTCGGTTCGAA	AGAGAGTCCTATCATTTCATCGAC	50.0	41.7	58.0	57.7	220 bp	1.0	47.2
trnL-UAG-ccsA_p2	CTTGAGCATCTCGGTTCGAA	GAGAGTCCTATCATTTCATCGACT	50.0	41.7	58.0	57.7	219 bp	1.0	47.2
trnL-UAG-ccsA_p3	CTTGAGCATCTCGGTTCGAA	AGAGTCCTATCATTTCATCGACT	50.0	39.1	58.0	56.6	218 bp	1.0	46.8
trnL-UAG-ccsA_p4	TAGACACGCTGCTCTTAGGA	AGAGAGTCCTATCATTTCATCGAC	50.0	41.7	57.9	57.7	247 bp	1.0	46.0
trnL-UAG-ccsA_p5	TAGACACGCTGCTCTTAGGA	GAGAGTCCTATCATTTCATCGACT	50.0	41.7	57.9	57.7	246 bp	1.0	46.0