Hotspot

trnG-GCC-trnR-UCU

LSC · 9904-10134 · 231 bp

Back to Hylotelephium

Pi 0.0145

MarkerSeek score 50.80

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 11
pi: 0.0145
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.2	55.6	317-320 bp	1.0	48.6
trnG-GCC-trnR-UCU_p2	TCCTGAGTTGACGTCGACTA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.8	55.6	344-347 bp	1.0	48.5
trnG-GCC-trnR-UCU_p3	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.2	54.6	311-314 bp	1.0	48.4
trnG-GCC-trnR-UCU_p4	TCCTGAGTTGACGTCGACTA	AGAAGACCTATGTCCTATCCA	50.0	42.9	57.8	54.6	338-341 bp	1.0	48.3
trnG-GCC-trnR-UCU_p5	TCCTGAGTTGACGTCGACTA	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	57.8	57.6	349-352 bp	1.0	47.8