Hotspot

ndhD

SSC · 120631-122133 · 1503 bp

Back to Hylomecon

Pi 0.00599

MarkerSeek score 55.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 9
pi: 0.00599
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGTCCAACGAACGATTTTGA	TCTTTACCGACAAAACCCCG	40.0	50.0	55.9	58.1	1656 bp	1.0	62.9
ndhD_p2	TGTCCAACGAACGATTTTGA	AAACCCCGCACTCGATAAAA	40.0	45.0	55.9	57.8	1644 bp	1.0	62.4
ndhD_p3	TGTCCAACGAACGATTTTGA	TATTTCGAGCACGGGGTTTT	40.0	45.0	55.9	57.8	1616 bp	1.0	62.4
ndhD_p4	TGTCCAACGAACGATTTTGA	TTATTTCGAGCACGGGGTTT	40.0	45.0	55.9	57.8	1617 bp	1.0	62.4
ndhD_p5	TGTCCAACGAACGATTTTGA	TTTATTTCGAGCACGGGGTT	40.0	45.0	55.9	57.8	1618 bp	1.0	62.4